Oct. 15, 2020
Oct. 13, 2020
Analysis of the root system is important in ensuring sustainable crop production, reducing nutrient input and irrigation, and protecting soil carbon pools. Getting rapid and frequent images of what is happening underground can help people make timely decisions about agricultural practices to maintain plant health and ensure the judicious use of resources. Root analysis can reduce financial and resource investments to make farming more profitable and environmentally friendly.
Root analysis is important not only to improve and increase food production but also for environment protection and fighting climate change.
In order to perform all their tasks, root systems have developed different morphology, topology, distribution, and architecture. Each root system is adapted for its longevity, region, and soil type.
Figure 1. Different root architecture in European dicots, from Kutschera and Lichtenegger (1992), and Lynch (1995)
1- Fryngium campestre; 2- Scorzonera. villosa; 3- Chondrilla juncea; 4- Pulsatilla pratensis; 5- Genista germanica; 6 -Trigonella balansae; 7-Trifolium trichocephalum; 8- Carum caucasicum; 9- Onosma arenarium; 1O- Silene otites.
The main disciplines using root analysis are concerned in some way with crop production, silviculture, and ecology. They are:
Agronomy: Root analysis helps in reducing abiotic and biotic stress in order to increase crop production. Anaylsis includes monitoring roots for efficient use of water and nutrients, plant health, and symbiosis. Root analysis is important for growers as well as agronomists.
Soil Science: The activity of roots influence soil, just as soil affects roots. Roots’ presence, respiration, activity, and exudates all create soil-root interactions that have been getting more attention from scientists in the past three decades.
Ecophysiology: Root ecophysiology, the study of internal and external factors on roots, is another area that has been getting the attention of scientists as of late. The functional, molecular, and physiological mechanisms of root systems and interactions with microbes and other roots are some of the new areas of interest.
Climatology: More carbon is added to the soil by roots than by litter deposition of the shoot system. Moreover, this carbon is likely to remain longer in the soil than the organic matter formed by shoot deposits. Root carbon is now recognised as a major carbon pool that needs to be protected and built up to fight climate change. So, root analysis is very important for scientists, international organisations, national governments, and local groups involved in climate change mitigation and adaptation.
Hydrology: Scientists are following the relationship of root depth with soil water availability in natural systems. Root architecture depends on hydrology and will differ in places with drought compared to plants growing in wetlands, for example.
It is difficult to see how roots fare and function underground. By the time symptoms are visible above-ground, it may be too late to fix the problem. Root imaging provides an excellent solution to this problem.
Roots face several challenges in their search for resources:
Agriculturists use root analysis to get images of:
Figure 2: Distribution of root density for different maturing crops, from Gregory 2006
In the past, the methods used to get a picture of the root system involved destructive methods such as coring or trenching. These techniques have the added disadvantage of being one-time observations. Current methods involve making 2D and 3D images of the roots systems, which are non-destructive, and can, therefore, take repeated pictures at the same spot. Root imaging can be done in real time, and as often as necessary depending on the objectives of the study.
Some of the common techniques that can be used in the field in situ are:
CID Bio-Science offers the CI-600 In-Situ Root Imager and CI-602 Narrow Gauge Root Imager, which are examples of the minirhizotron system. They provide high resolution digital images that track the development and functionality of the roots down to a depth of two meters.
Root analysis through imaging is a relatively new field technique. It was previously used only in laboratories for phenotyping roots and other studies on root systems. Its recent application in the field, especially in crop production – broad acre and orchards – is making agriculture more sustainable. It is suitable for emerging forms of cultivation such as Adaptive Farming and Precision Farming to cut costs and optimize resource use.
Science Writer, CID Bio-Science
Ph.D. Ecology and Environmental Science, B.Sc Agriculture
Bucksch, A., Burridge, J., York, L.M., Das, A., Nord, E., Weitz, J.S., & Lynch, J.P. (2014). Image-Based High-Throughput Field Phenotyping of Crop Roots. Plant Physiology 166 (2) 470-486.doi: 10.1104/pp.114.243519
Ecophysiology of root systems-environment interactions. Retrieved from https://www.frontiersin.org/research-topics/1043/ecophysiology-of-root-systems-environment-interactions
Gregory, P.J. (2006). Roots, rhizosphere and soil: the route to a better understanding of soil science? European Journal of Soil Science 57. https://doi.org/10.1111/j.1365-2389.2005.00778.x
Leitner, D., Felderer, B., Vontobel, P., & Schnepf, A. (2013). Recovering root system traits using image analysis exemplified by two-dimensional neutron radiography images of lupine. Plant Physiology, 164(1) 24–35. doi:10.1104/pp.113.227892
Lynch J. (1995). Root Architecture and Plant Productivity. Plant Physiol. 109: 7-13. Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC157559/pdf/1090007.pdf
Malhotra, A., D. Sihi, and C. M. Iversen (2018). The fate of root carbon in soil: data and model gaps, Eos 99. https://doi.org/10.1029/2018EO112593.
Paya, A.M., Silverberg, J.L., Padgett, J., & Bauerle, T.L. (2015). X-ray computed tomography uncovers root–root interactions: quantifying spatial relationships between interacting root systems in three dimensions. Front. Plant Sci. https://doi.org/10.3389/fpls.2015.00274
Rewald, Boris & Ephrath, Jhonathan. (2013). Minirhizotron Techniques. doi: 10.1201/b14500-50
Rutgers University. (2017, September 18). Deep roots in plants driven by soil hydrology. Retrieved from https://phys.org/news/2017-09-deep-roots-driven-soil-hydrology.html
Schulz H., Postma J.A., van Dusschoten D., Scharr H., Behnke S. (2013) Plant Root System Analysis from MRI Images. In: Csurka G., Kraus M., Laramee R.S., Richard P., Braz J. (eds) Computer Vision, Imaging and Computer Graphics. Theory and Application. Communications in Computer and Information Science 359. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-38241-3_28
Van Dusschoten, D., Metzner, R., Kochs, J., Postma, J.A., Pflugfelder, D., Bühler, J., Schurr, U., & Jahnke, S. (2016). Quantitative 3D Analysis of Plant Roots Growing in Soil Using Magnetic Resonance Imaging. Plant Physiology 170:1176-1188. doi: 10.1104/pp.15.01388
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