Plant Science Tools

CI-710s

SpectraVue Leaf Spectrometer


Non-destructive, rapid measurement of plant stress & pigments

The CI-710s SPECTRAVUE Leaf Spectrometer is designed to simultaneously measure the transmission, absorption and reflection of light by biological substances within a wide range of wavelengths that cover visible and Near Infra-Red (NIR) light. The spectrum can be used for the quantification of chemical concentrations, color analysis, the study of photochemical reactions, and the quantification of physical or optical properties such as film thickness, index of refraction, and extinction coefficient.

The newly redesigned SPECTRAVUE Leaf Spectrometer has been upgraded with an all new spectrometer (360-1100 nm) to allow full operation in a handheld form factor with a 7” 1024 x 600 IPS touchscreen display.  This allows easy portability for remote operation with a full suite of built in analysis software.

Click here for a downloadable compilation of published research.

Specifications

Dimension: 220 mm x 150 mm x 30 mm

Weight: 952 g

Operating Environment: -30° to 70° C storage, -10° to 50° C Operation,
0% - 90% noncondensing humidity

Minimum Leaf Size: 20 mm x 20 mm

Display: 7” 1024 x 600 IPS Display

Languages: English, Spanish

Measure modes: Reflectance, Transmittance and Absorbance



Detector Specifications

Detector: CMOS Linear Array

Wavelength Range: 360 - 1100 nm 

Pixels: 2048 pixels

Pixel Size: 14 μm x 200 μm

Pixel Well Depth: 100,000 electrons

Signal-To-Noise Ratio: 330:1 (at full signal)

A/D Resolution 16 bit

Dark Noise: 16 counts

Corrected Linearity: >99.8%

Sensitivity: 337.500

Wavelength Data Increment: 0.55 - 0.7 nm



Spectroscopic

Grating: 300 lines/mm, Slit = 55 μm

Optical Resolution: 2.4 FWHM in nm

Integration Time: 30 μs – 60 seconds

Dynamic Range: 3300:1

Stray Light: 0.2 – 1.0%



Electronics

Power Supply: Two 18650 batteries and USB-C

Battery life: 3 - 4 hours

Trigger Modes

Automatic & Manual


Built-in Indices

Abbrev Index

ARI1

Anthocyanin Reflectance Index 1

ARI2

Anthocyanin Reflectance Index 2

CRI1

Carotenoid Reflectance Index 1

CRI2

Carotenoid Reflectance Index 2

SIPI

Structure Intensive Pigmane Index

FRI

Flavonols Reflectance Index

CNDVI

Chlorophyll Normalized Difference Vegetation Index

Ctr1

Carter Index 1

Ctr2

Carter Index

G

Greeness Index

GM1

Gitelson and Merzlyak Index 1

GM2

Gitelson and Merzlyak Index 2

Lic1

Lichtenthaler Index 1

Lic2

Lichtenthaler Index 2

MCARI1

Modified Chlorophyll Absorbtion Ratio Index 1

MCARI

Modified Chlorophyll Absorbtion Ratio Index 2

NDVI

Normalized Difference Vegetation Index

NPCI

Normalized Pigment Chlorophyll Index

NPQI

Normalized Phaeophytinization Index

SRPI

Simple Ratio Pigment Index

TCARI

Transformed CAR Index

TVI

Triangular Vegetation Index

ZMI

Zarco-Tejada & Miller Index

MDATT

Modified DATT Index

CCI

Chlorophylll Content Index

IAD

Absormance Difference Index

CPHLA

Chlorophyll A (ug/cm^3)

CPHLB

Chlorophyll B (ug/cm^3)

CPHLT

Chlorophyll Total (ug/cm^3)

SPAD

Soil-Plant Analysis Development

Grape

Grape PLS Analysis

Spinach

Spinach PLS Model

MRESRI

Modified Red Edge Simple Ratio Index

RENDVI

Red Edge Normalized Difference Vegetation Index

VREI1

Vogelmann Red Edge Index 1

VREI2

Vogelmann Red Edge Index 2

VREI3

Vogelmann Red Edge Index 3

PRI

Photochemical Reflection Index

PSRI

Plant Senescence Reflectance Index

WBI

Water Band Index

Theory of Operation

SPECTRAVUE is a powerful spectrometer paired with a leaf probe attachment, on-board operating software, and display screen. Two broadband light sources are attached inside the device. One is positioned in the leaf clamp for transmissive measurements, and one is placed inside the case for reflective measurements. The spectrometer module takes the light from the leaf probe attachment and projects the wavelength dispersed light onto a CCD array. Each pixel of the CCD array thus corresponds to a specific wavelength of light. The operating software displays the light intensity of each pixel of the CCD array.

Three spectroscopic measurements can be performed by SPECTRAVUE:

Transmittance (T) is the fraction of the incident light passing through a sample, such as a leaf. When the intensity of the incident light is Io, and the intensity of light passing through the sample is It, the transmittance, T, is then expressed as T = It / Io. The CI-710s software suite can automatically calculate and plot the transmission spectrum with the reference spectrum (the intensity profile of the light source) stored.

Absorbance (A) is related to the transmittance as A = - log T = -log(It / Io). Absorbance is commonly denoted in optical density (OD) as it is linearly proportional to the amount of light absorbing sample in the light path length (Beer-Lambert law). For example, in a standard 1-cm cuvette, a sample with an OD of 2 is twice as concentrated as a sample with an OD of 1. But note that the intensity of light transmitted from a sample with an OD of 2 is only 1/10 of that from a sample with an OD of 1. Transmission and absorption spectrum measurements are the most common applications of spectrometers. The spectrum can be used for the quantification of chemical concentrations, color analysis, the study of photochemical reactions such as photosynthesis, and the quantification of physical or optical properties such as film thickness, index of refraction, and extinction coefficient.

Reflectivity (R) is the fraction of incident light that is reflected from a sample. Depending on the purposes of the study, reflectivity can be measured for reflection at a specific angle from the sample, or the entire reflected hemisphere. The leaf probe attachment of CI-710s is not designed to measure the directional reflectivity. Nonetheless, it can be used to measure the absorption spectrum of surface substance of reflective or opaque samples.

Features

  • Wide range spectrum (360-1100 nm)
  • On-board analysis software
  • 7" 1024x600 display
  • High resolution and real-time high speed scanning
  • Very high sensitivity; ideal for fluorescence and other low light level applications
  • USB interface
  • GPS enabled
  • In-field analysis
  • Simultaneous measurement of absorbance, reflectance and transmittance
  • Integrating time = 3.8 ms - 10 seconds
  • Lightweight and fully portable